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| 106. |  | BEZERRA NETO, F.; SALDANHA, T. R. da C.; NEGREIROS, M. Z. de; OLIVEIRA, A. M. de. Avaliação da competição intra e inter-específica em fileiras de cenoura consorciadas com quatro cultivares de alface crespa em dois sistemas de cultivo em faixas. Horticultura Brasileira, Brasília, v. 20, n. 2, jul. 2002. Suplemento 2. Trabalho apresentado no 42º Congresso Brasileiro de Olericultura, 2002. Publicado também como resumo em: Horticultura Brasileira, Brasília, v. 20, n. 2, p. 301, jul. 2002. Suplemento 1.| Biblioteca(s): Embrapa Hortaliças. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
30/07/2020 |
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Data da última atualização: |
25/08/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
IWANICKI, N. S.; MASCARIN, G. M.; MORENO, S. G.; EILENBERG, J.; DELALIBERA JUNIOR, I. |
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Afiliação: |
NATASHA SANT'ANNA IWANICKI, ESALQ-USP; GABRIEL MOURA MASCARIN, CNPMA; SARA GIRO MORENO, ESALQ-USP; JORGEN EILENBERG, University of Copenhagen; ITALO DELALIBERA JUNIOR, ESALQ-USP. |
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Título: |
Growth kinetic and nitrogen source optimization for liquid culture fermentation of Metarhizium robertsii blastospores and bioefficacy against the corn leafhopper Dalbulus maidis. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
World Journal of Microbiology and Biotechnology, v. 36, article 71, 2020. |
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ISSN: |
1573-0972 |
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DOI: |
https://doi.org/10.1007/s11274-020-02844-z |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The cosmopolitan entomopathogenic and root endophytic fungus Metarhizium robertsii has a versatile lifestyle and during liquid fermentation undergoes a dimorphic transformation from hyphae to conidia or microsclerotia, or from hyphae to blastospores. In all cases, these processes are mediated by environmental and nutritional cues. Blastospores could be used in spray applications to control arthropod pests above ground and may serve as an attractive alternative to the traditional solid-grown aerial conidial spores of Metarhizium spp. found in commercial products. Nitrogen is a vital nutrient in cell metabolism and growth; however, it is the expensive component in liquid cultures of entomopathogenic fungi. Our goals in this study were to optimize nitrogen sources and titers for maximum production of M. robertsii blastospores cultured in shake flasks at highly aerated conditions and to further determine their virulence against the corn leafhopper Dalbulus maidis, an important vector of serious pathogens in maize crops worldwide. Our fermentation studies revealed that the low-cost corn steep liquor (CSL) was the most suitable nitrogen source to improve blastospore growth in M. robertsii.The growth kinetic assays determined the optimal titer of 80 g L-1 and a yield up to 4.7 × 100.000.000 cells mL-1 within 5 days of cultivation (3 days preculture and 2 days culture), at a total cost of US$0.30 L-1. Moreover, the blastospore growth kinetic was strongly dependent on glucose and nitrogen consumptions accompanied by a slight drop in the culture pH. Insect bioassays evidenced a high virulence of these blastospores, either as dried or fresh cells, to D. maidis adults fed on maize plants. Our findings provide insights into the nutritional requirements for optimal and cost-efficient production of M. robertsii blastospores and elucidate the potential of blastospores as an ecofriendly tool against the corn leafhopper. MenosAbstract: The cosmopolitan entomopathogenic and root endophytic fungus Metarhizium robertsii has a versatile lifestyle and during liquid fermentation undergoes a dimorphic transformation from hyphae to conidia or microsclerotia, or from hyphae to blastospores. In all cases, these processes are mediated by environmental and nutritional cues. Blastospores could be used in spray applications to control arthropod pests above ground and may serve as an attractive alternative to the traditional solid-grown aerial conidial spores of Metarhizium spp. found in commercial products. Nitrogen is a vital nutrient in cell metabolism and growth; however, it is the expensive component in liquid cultures of entomopathogenic fungi. Our goals in this study were to optimize nitrogen sources and titers for maximum production of M. robertsii blastospores cultured in shake flasks at highly aerated conditions and to further determine their virulence against the corn leafhopper Dalbulus maidis, an important vector of serious pathogens in maize crops worldwide. Our fermentation studies revealed that the low-cost corn steep liquor (CSL) was the most suitable nitrogen source to improve blastospore growth in M. robertsii.The growth kinetic assays determined the optimal titer of 80 g L-1 and a yield up to 4.7 × 100.000.000 cells mL-1 within 5 days of cultivation (3 days preculture and 2 days culture), at a total cost of US$0.30 L-1. Moreover, the blastospore growth kinetic was strongly dependent on gluco... Mostrar Tudo |
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Palavras-Chave: |
Biocontrol; Dimorphic growth; Fungal entomopathogen; Fungal nutrition. |
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Thesagro: |
Fungo Para Controle Biológico; Milho; Praga de Planta; Zea Mays. |
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Thesaurus NAL: |
Biological control agents; Corn; Entomopathogenic fungi; Metarhizium robertsii; Plant pests; Virulence. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
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001 2124093
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008 2020 bl uuuu u00u1 u #d
022 $a1573-0972
024 7 $ahttps://doi.org/10.1007/s11274-020-02844-z$2DOI
100 1 $aIWANICKI, N. S.
245 $aGrowth kinetic and nitrogen source optimization for liquid culture fermentation of Metarhizium robertsii blastospores and bioefficacy against the corn leafhopper Dalbulus maidis.$h[electronic resource]
260 $c2020
520 $aAbstract: The cosmopolitan entomopathogenic and root endophytic fungus Metarhizium robertsii has a versatile lifestyle and during liquid fermentation undergoes a dimorphic transformation from hyphae to conidia or microsclerotia, or from hyphae to blastospores. In all cases, these processes are mediated by environmental and nutritional cues. Blastospores could be used in spray applications to control arthropod pests above ground and may serve as an attractive alternative to the traditional solid-grown aerial conidial spores of Metarhizium spp. found in commercial products. Nitrogen is a vital nutrient in cell metabolism and growth; however, it is the expensive component in liquid cultures of entomopathogenic fungi. Our goals in this study were to optimize nitrogen sources and titers for maximum production of M. robertsii blastospores cultured in shake flasks at highly aerated conditions and to further determine their virulence against the corn leafhopper Dalbulus maidis, an important vector of serious pathogens in maize crops worldwide. Our fermentation studies revealed that the low-cost corn steep liquor (CSL) was the most suitable nitrogen source to improve blastospore growth in M. robertsii.The growth kinetic assays determined the optimal titer of 80 g L-1 and a yield up to 4.7 × 100.000.000 cells mL-1 within 5 days of cultivation (3 days preculture and 2 days culture), at a total cost of US$0.30 L-1. Moreover, the blastospore growth kinetic was strongly dependent on glucose and nitrogen consumptions accompanied by a slight drop in the culture pH. Insect bioassays evidenced a high virulence of these blastospores, either as dried or fresh cells, to D. maidis adults fed on maize plants. Our findings provide insights into the nutritional requirements for optimal and cost-efficient production of M. robertsii blastospores and elucidate the potential of blastospores as an ecofriendly tool against the corn leafhopper.
650 $aBiological control agents
650 $aCorn
650 $aEntomopathogenic fungi
650 $aMetarhizium robertsii
650 $aPlant pests
650 $aVirulence
650 $aFungo Para Controle Biológico
650 $aMilho
650 $aPraga de Planta
650 $aZea Mays
653 $aBiocontrol
653 $aDimorphic growth
653 $aFungal entomopathogen
653 $aFungal nutrition
700 1 $aMASCARIN, G. M.
700 1 $aMORENO, S. G.
700 1 $aEILENBERG, J.
700 1 $aDELALIBERA JUNIOR, I.
773 $tWorld Journal of Microbiology and Biotechnology$gv. 36, article 71, 2020.
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| 1. | | 1100056, CANADIAN PLANT DISEASE SURVEY, Research Branch Agriculture Canada, Ottawa-Canada
Biblioteca(s): Catálogo Coletivo de Periódicos Embrapa; Embrapa Agropecuária Oeste; Embrapa Arroz e Feijão; Embrapa Cerrados; Embrapa Clima Temperado; Embrapa Hortaliças; Embrapa Trigo; Embrapa Uva e Vinho. | |
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